Fig. 5

Immunophenotypic profiles of NK-like cells derived from WT and CAR-TIM3 iPSCs. A Flow cytometric analysis of typical NK surface markers CD16, CD56, and NK cell-related receptors, including CD94, CD158, CD161, Nkp30, Nkp44, Nkp46 and NKG2D on NK-like cells derived from parental WT- and CAR-TIM3 iPSCs on day 30 of culture. B Flow cytometric analysis of CD3 and CD56 confirms the absence CD3⁺ CD56⁻ T-like and CD3⁺ CD56⁺ NKT-like cells. C Anti-TIM3 CAR expression in the differentiated CAR-TIM3 NK-like cells as evaluated by flow cytometry based on its binding activity to specific antigen His tag-rhTIM3 (23–200)