Fig. 6

CAR-TIM3 NK-like cells selectively induced cell death of TIM3-positive target leukemia cells. A Schematic diagram of NK cytotoxicity assay showing the labeling of target tumor cells with PKH67 dye before exposure to effector cells at various E:T ratios. B Flow cytometric gating strategy to specifically detect cell death in PKH67-labeled target tumor cells and NK cell markers in effector NK-like cells. C Percentages of total tumor cell death, comprising Annexin V- and/or 7-AAD-positive cells, in TIM3-negative K562 cells and TIM3-positive KG-1 cells and TIM3-overexpressed U937 cells at indicated E:T ratio were plotted. Data are mean ± SEM (n = 3). *p < 0.05; **p < 0.01; and ***p < 0.001 versus WT NK-like cells in the same condition; unpaired t-test