Fig. 6

Inhibition of Tiam1 expression in pancreatic cancer (PC) by the inhibitors of glucose metabolism. A Glucose uptake, lactate production, and ATP levels were measured in PC cell lines. B Colony formation assay indicated that 2-deoxy-d-glucose (2-DG) or 3-bromopyruvate (3BrPA) inhibited Tiam1 overexpression-induced cell proliferation. C 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and D Matrigel tube formation assays. E Western blotting. F Transwell assay. G Wound healing assay. In all panels, data are presented as the mean ± standard deviation (SD). *p < 0.05, **p < 0.01