Fig. 5

Robinin (1 µM/mL) alleviated EMT and inflammation in Mia-PACA2 and PANC-1. (A) qRT-PCR of α-SMA and snail expression in Mia-PACA2 and PANC-1. (B) qRT-PCR of IL-6 and TNF-α expression in Mia-PACA2 and PANC-1. (C-D) Protein levels of EMT and inflammation in Mia-PACA2 and PANC-1 were analyzed by western blot analysis and normalized to β-actin expression. P < 0.05, **P < 0.01, vs. the control group, #P < 0.05, ##P < 0.01, vs. the Robinin co-cultivated with CU-T12-9 group. Measurement data were expressed as mean ± standard derivation; data between PC cell and HPNE were compared by t-test, and measurement data among multiple groups were analyzed by one-way analysis of variance with Tukey’s post hoc test. The experiment was repeated three times