Fig. 1

Knockdown of PRRG4 decreases the mRNA levels of mtDNA protein-encoding genes. A A Venn diagram showing the overlap of DEGs between SKBR3 cells expressing two different PRRG4-shRNAs (sh94 and sh95) compared with the control-shRNA. B, C GO and KEGG analyses demonstrated that DEGs were significantly enriched in electron transport chain and oxidative phosphorylation. D Heat map of RNA-seq data showing the mRNA levels of mitochondrial protein-encoding genes in SKBR3 cells expressing two different PRRG4-shRNAs (sh94 and sh95) and control-shRNA. PRRG4-sh94 group compared with control-shRNA group, **p < 0.01, ***p < 0.001; PRRG4-sh95 group compared with control-shRNA group, ^##p < 0.01, ^###p < 0.001, ns p > 0.05. E PRRG4 knockdown reduces the transcript levels of mitochondrial protein encoding genes. Total RNAs were isolated from SKBR3 and SUM149PT cells expressing control-shRNA and two different PRRG4-shRNAs (sh94 and sh95), reverse-transcribed, and subjected to q-PCR to quantitate the relative levels of transcripts for the indicated mtDNA-encoded genes. *p < 0.05, **p < 0.01, ***p < 0.001