Fig. 2

PRRG4 regulates mtDNA content and mitochondrial function. A Knockdown of PRRG4 with shRNAs decreases mtDNA content in SKBR3 and SUM149PT cells. B Overexpression of PRRG4 increases mtDNA content in MDA-MB-231 and HCC1954 cells. C and D The basal ATP content (Base) and oligomycin-sensitive ATP content (Oligo-sensitive) in SKBR3 and SUM149PT with PRRG4 knockdown, and MDA-MB-231 and HCC1954 with PRRG4 overexpression were measured using a luciferin/luciferase chemiluminescence ATP assay kit. Cells were treated with 1 µg/mL oligomycin for 1 h. *p < 0.05, **p < 0.01, ***p < 0.001