Fig. 1

The overall experimental design and major steps of our study. We first investigated the expression of 16 disulfidptosis-related genes in HCC patients in the TCGA-LIHC cohort and categorized the HCC patients into two DRG clusters based on the expression of those genes. Subsequently, we selected DEGs with prognostic significance between DRG clusters for LASSO analysis. In total, 19 hub genes were selected for the construction of the DRG score model in the TCGA cohort, which was validated in an external ICGC cohort. Then, we developed a nomogram based on our model and assessed immune infiltration and drug sensitivity in HCC patients. Finally, through experimental validation, we confirmed the anticancer role of the hub gene LCAT in liver cancer and explored its potential association with disulfidptosis-related death. DRG Disulfidptosis-related gene, HCC hepatocellular carcinoma, LASSO least absolute shrinkage and selection operator, DEG differentially expressed gene