Fig. 2
From: Navigating challenges: optimising methods for primary cell culture isolation
Stages of colony formation. Stage I: A piece of tumour tissue attaches to the plate; it is convex and looks more “3D-like”. Stage II: The cells’ increasing flattening and mobility are observed after attachment to the plate. Densely growing cells are visible in the colony’s centre, and spread cells at the edges. Stage III: A primary cell culture is obtained. Values are averaged based on our observations