Fig. 4
From: Navigating challenges: optimising methods for primary cell culture isolation
The changing phenotype of cell lines after passaging. Due to the heterogenic composition of cells in the BC tissue, the percentage of different cell populations varies. During the isolation process, the passage caused the reduction of the population of neoplastic cells, resulting in the derivation of the CAF cell line. Cells were stained with CD90, CD24 and CD44 and assessed with flow cytometry