Table 1 Comparison of evaluated methods and condition of isolated cell cultures
From: Navigating challenges: optimising methods for primary cell culture isolation
Property | Method 1 | Method 2 | Method 3 | Method 4 | Method 5 |
|---|---|---|---|---|---|
Days until tissue attachment | 1–2 | - | 3–5 | - | 1–2 |
Days until colony formation | 18–21 | - | 28 | - | 10–21 |
Number of colonies formed per well | 7–10 | - | 2–3 | - | 7–12 |
Cell culture lifespan (number of passage) | P7-P10 | - | P5 | - | P7-P10+ |
Type and number of isolated cells per trial | CAFs (3) | - | CAFs (2) | - | BC cells (1), CAFs (2) |