Table 2 Different media compositions for isolation of primary cell lines from tumours
From: Navigating challenges: optimising methods for primary cell culture isolation
Type of medium | Serum | Antibiotics | Growth factors | Hormones | Other | References |
|---|---|---|---|---|---|---|
DMEM/F12 (1:1) | 10% foetal calf serum (FCS) | 50 µg/mL penicillin, 0.1 mg/mL streptomycin, 2.5 µg/mL amphotericin-B, 1 µg/mL minocycline | 10 ng/mL EGF | 1 µg/mL insulin, 1 µg/mL hydrocortisone | 10 µg/mL transferrin, 11 µg/mL ethanolamine, 50 ng/mL cholera toxin | [22] |
DMEM/F12 | 10% FBS | 1% penicillin/streptomycin | - | - | - | [23] |
F12/DMEM (3:1) | FBS | - | EGF | hydrocortisone, insulin | cholera toxin, adenine, Rho-associated protein kinase (ROCK) | [24] |
IMDM + epithelial cell growth supplement (EpiCGS) | 10% FBS | 100 U/mL penicillin, 100 µg/mL streptomycin, 250 mg/ mL amphotericin-B | EGF | - | 10 µM ROCK, 2 mM L-glutamine | [25] |
DMEM/F12 (1:1) | 10% FCS | 100 UI/ ml penicillin, 100 mg/ml streptomycin | - | 2 mg/mL bovine insulin + 10 nM estradiol, 0.3 mM cortisol, 10 nM triiodothyronine, 10 ng/ml transferrin | 2 mM glutamine | [26] |
DMEM | 10–20% FBS | - | 5–15 ng/ml EGF | 100 U/ml insulin | 2 mM glutamine | [6] |
DMEM/F12 + Geltrex®, collagen I or feeder layer | 2% human serum | 1% penicillin, streptomycin, 0.2% gentamycin | 10 ng/ml EGF | 5 µg/ml insulin, 0.32 µg/ml hydrocortisone | 20 µg/ml adenine, 8.4 ng/ml CHTX, 15mM HEPES, 10 µM ROCK | [21] |