Fig. 7

AAA237 inhibited the proliferation of GBM in LN229 orthotopic model. A The flow chart of LN229 orthotopic model. B Representative MRI images of intracranial tumors from various groups of the LN229 orthotopic model (Scale bar = 2 mm). C Tumor volumes in the LN229 orthotopic model. D Relative brain weight in the LN229 orthotopic model. E Changes in body weight during the AAA237 administration period. F AAA237 had no side effects on the major organs of the test mice. G H&E staining and immunohistochemical results in brain tissues of GBM orthotopic nude mice model. Scale bar = 50 μm. Data are presented as mean ± SD (n = 6). Statistical significance was determined by one-way ANOVA, ***P < 0.001 vs. Vehicle. H Expression of SKP2, P27 and P21 in mouse tumor tissue was checked by Western blot under treatment with low and high concentrations of AAA237 (15 mg/kg and 45 mg/kg). I Expression of BNIP3 in mouse tumor tissue was checked by Western blot under treatment with low and high concentrations of AAA237 (15 mg/kg and 45 mg/kg). J Expression of p-mTOR, mTOR, Beclin 1, ATG5 and LC3II in mouse tumor tissue was checked by Western blot under treatment with low and high concentrations of AAA237(15 mg/kg and 45 mg/kg)