Fig. 9

Effect of DA and ATZ on cell morphology of SKBR3 and ZR75, after 48 h (a). In controls, SKBR3 and ZR75 cells have round or polygonal morphology and form clusters. Upon treatment, we notice smaller clusters and cell death.(images a and b at ×20 magnification). The outcome of DA and ATZ on colony formation of SKBR3 and ZR75 cell lines in soft agar after 21 days (b & c). DA and ATZ inhibit colony formation of SKBR3 and ZR75 in comparison with their matched control cells. Colonies were counted manually and expressed as a percentage of treatment relative to the control (Mean ± SEM)