Fig. 3

Confocal microscopy of ATP1A1 in melanoma cells. A, B. Immunofluorescence images of ATP1A1 (red) and Cav1 (green) in HBL cells (nucleus in blue); a colocalisation of ATP1A1 and Cav-1 is observed in 2D (A) and 3D (B) images. C The colocalization was confirmed by PLA; the green spots indicate a physical interaction between the two proteins, nuclei are stained in blue with DAPI whereas phalloidin was used to stain cell cytoplasm in red. Scale bars = 5 µm)