Fig. 5

Effect of bufalin on SRC activity and signaling pathways. A Western blot analysis and quantification of the active form of SRC (phosphorylation 416) and the inactive form (phosphorylation 527) in melanoma cells (sensitive and resistant (-R)) treated with 10 nM bufalin (BUFA) for 24 h, data are presented in mean + SD, ANOVA and Tukey post-hoc test. B Phosphorylation profiles in melanoma cell lines; heatmap showing the phosphorylation level of 37 proteins according to the cell lines and the treatment (10 nM bufalin for 30 min vs untreated). Shades of red represents a high phosphorylation profile while shades of blue correspond to a weak phosphorylation expression. C Signaling network including the most significant correlation with ATP1A1 in relation with bufalin treatment (STRING)