Fig. 6
Effect of bufalin in nude mice xenografted with MM074 or MM074-R cell lines, 6 mice were for each experimental condition. A No effect of stably deliver 2 mg/kg/d of bufalin to the mice for 14 days regarding to heart, liver, spleen, kidney and lung tissue morphology. B No side effect of bufalin evaluating troponin I staining in hearts of control mice and bufalin treated mice (Control + is a heart of a mouse treated with silica to induce an autoimmune myocarditis). Scale bars in B, C = 50 µm. C Effect of bufalin on tumor volume developed from MM074 cells in nude mice. D Effect of bufalin on tumor volume growing from MM074-R cells in nude mice. Data C, D are presented in mean + SD, ANOVA and Tukey post-hoc test. E Box plot for MM074 cells presenting tumor volume and weight after mouse euthanasia in both control and bufalin-treated groups. F Box plot for MM074-R cells showing tumor volume (mm3) and weight (g) after mouse euthanasia in both control and bufalin-treated groups (Mann Whitney test)