Fig. 2

Construction and validation of the necroptosis-related prognostic gene signature. The normalized expression profiles of hub NRGs (A). The necroptosis score distribution and survival status of patients in the training cohort (B). PCA results for two groups of patients in the training cohort (C). Kaplan-Meier survival analysis of patients in the training cohort (D) and validation cohort (E). COX regression showed that necroptosis score was a prognostic factor independent of other clinical characteristics (F). Receiver operating characteristic curves of the necroptosis score score in distinguishing patient prognosis (G). The nomogram consisted of gender, age and necroptosis score based on the five-NRG signature (H). The calibration curves for internal validation of the nomogram for estimating the survival of osteosarcoma patients at 3 and 5 years (I). Decision curve analysis indicated a greater net benefit of nomogram (J)