Fig. 5

Analysis of single-cell data of osteosarcoma. Uniform manifold approximation and projection plots show eight main cell types in single-cell data (GSE162454), colored by cell type (A) and the NS (B). The average NS (C) and normalized expression of hub gene (D) in eight main cell types. Bubble chart showing significant ligand–receptor interactions between high-NS/low-NS malignant cells (malignant H/malignant L) and neighboring cells. Ligand-expressed cells and receptor-expressed cells are shown on the x-axis, ligands and receptors are shown on the y-axis. The color indicates the average expression levels of ligands and receptors in interacting cells, and the bubble size indicates the significance of the interactions (E)