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Fig. 8 | Cancer Cell International

Fig. 8

From: Identification of TNFRSF21 as an inhibitory factor of osteosarcoma based on a necroptosis-related prognostic gene signature and molecular experiments

Fig. 8

Overexpression of TNFRSF21 promoted necroptosis. TUNEL staining (red fluorescence) on cell apoptosis, nuclei were counterstained blue with DAPI (Scale bars, 50 μm) (A). Percentage of cells in the late stages of apoptosis was assessed via flow cytometry, P value was calculated by 2-tailed Student t test (n = 3)(**, P < 0.01; ***, P < 0.001;) (B). The phosphorylation levels of necroptosis mediators were detected by western blot (C). The activity of caspase 8 was assessed by measuring the optical density in 405 nm. Incremental activity of caspase 8 was expressed as a percentage of control, P value was calculated by 2-tailed Student t test (n = 3)(*, P < 0.05; ***, P < 0.001;) (D)

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Cancer Cell International

ISSN: 1475-2867

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