Fig. 5

Phenotypic assessment of prostate cancer cell nuclear stress responses upon platinum drug treatments. NPM and Coilin immunofluorescence staining -based quantitative analysis of the phenotypes of A LNCaP, B PC-3, and C 22Rv1 cells in response 24 h drug treatments. Scale bars, 5 µm. Left panels: example nuclei in the immunofluorescence staining. NPM (green), Fibrillarin (red), merge of NPM and Fibrillarin (Merge RG), brightfield, DAPI, and Merge of NPM, Fibrillarin and DAPI is shown. Right panels: t-sne of computational feature-based single-cell analysis showing phenotypic distributions of the drug responses in each cell line