Fig. 8

TDP-43 and FUS regulate Cajal body organization in prostate cancer cells. Knock-down of TDP-43 and FUS with siRNA targeting in LNCaP and PC-3 cells followed by immunofluorescence staining of TDP-43 or FUS and Coilin in the nuclei of the cells. Left panels: TDP-43 or FUS (green), Coilin (red), merge of TDP-43 or FUS with Coilin (Merge RG), brightfield, DAPI, and Merge of TDP-43 or FUS with Coilin and DAPI is shown. Scale bars, 5 µm. Right panels: Quantitation of CBs according to Coilin spot number in the siRNA-treated cells