Fig. 1

Adaptive stress response was activated in A2780 cells. A A2780 and SKOV3 cells were treated with 1.25–10 μM PKI-402 for 24 h and then cell viability was detected by MTT assays, and IC50 was calculated by GraphPad Prism 7.0. B A2780 and SKOV3 cells were treated with PKI-402 (2.5 μM) for 12 h and 24 h, respectively, and levels of apoptosis-associated proteins were detected by western blotting. D A2780 cells and SKOV3 cells were treated with PKI-402 (2.5 μM) for 12 h. Cell lysates were immunoblotted using the indicated antibodies. C, E, F. Levels of the proteins in B and D were measured by western blotting, respectively. Data were expressed as mean ± SEM of three independent experiments; ****P < 0.0001 compared to the control