Fig. 5
SGs regulate UPRmt by affecting the localization of ATF5. A A2780 cells were treated with PKI-402 (2.5 μM) for 12 h. Cell lysates were immunoblotted using the indicated antibodies. B, C. Levels of proteins in 6A were measured by Western blotting. D A2780 cells were treated with PKI-402 (2.5 μM) for 12 h. Cell lysates were obtained using anti-YB-1 and subjected to semiquantitative qPCR using G3BP1. E. Immunoprecipitation was performed using anti-G3BP1 antibody followed by Western blotting using anti-G3BP1 and anti-ATF5 antibodies. F Nuclear proteins from A2780 cells were collected after treatment with PKI-402 (2.5 μM) for 12 h and ATF5 was measured by Western blotting