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Fig. 6 | Cancer Cell International

Fig. 6

From: What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance

Fig. 6

GPR65 is downregulated in OS tumor tissues. (A) Representative images of IHC staining for GPR65 in a clinical sample tissue microarray. Magnification: 5თ(scale bar: 200 μm) and 40თ(scale bar: 20 μm). n = 15 per group. (B-C) Western blot (B) or qRT-PCR (C) analysis of GPR65 expression in U2OS and HOS cells transfected with Flag-GPR65 or siRNAs against GPR65 (siGPR65). (D-E) MTT assay detected the viability of OS cells with or without GPR65 overexpression (D) or knockdown (E). *p < 0.05 significantly different from control (siNC or Vector) group

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Cancer Cell International

ISSN: 1475-2867

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