Fig. 3

USP5 deficiency inhibits cell proliferation, viability and migration. A USP5 protein levels in WT and USP5-deficient T24 cells were measured by western blotting with GAPDH as a loading control. B Colony formation assays showed the viability of USP5-deficient T24 bladder cancer cells. Colonies were stained with crystal violet and subsequently imaged (left). The number of colonies was determined and plotted (right, n = 3). C Cell proliferation was analysed by CCK-8 assays with daily measurements for 5 d (n = 6). D Scratch assays were used to evaluate the effects of USP5 deficiency on T24 cells. The cells were imaged (left, ×20 magnification) and counted, and the results were plotted (right, n = 3). The data (mean ± SEM) are representative of 3 independent experiments. E Transwell experiments were used to evaluate the effects of USP5 deficiency on T24 bladder cancer cell migration. The cells were imaged (left, ×20 magnification) and counted, and the results were plotted (right, n = 3). The data (mean ± SEM) are representative of 3 independent experiments. Statistical significance was analysed by ANOVA or Student’s t test. *P < 0.05, ****P < 0.0001