Fig. 3
Functions of circRAD23B in patient-derived organoids (PDOs). (A) Immunofluorescence staining of primary tumours and PDOs, scale = 25 μm. (B) Organoids were transfected with sh-circRAD23B(or NC) and pLV-circRAD23B(or vector). (C) The size of organoids was observed in brightfield at different time pionts, scale = 25 μm. (D) Immunohistochemistry of Ki67 in PDOs. (E) Morphological changes of PDOs under 40 µM carboplatin pressure for 48 h. The red arrow indicates apoptotic cells, scale = 25 μm. (F) Organoids stained for cell viability/death; live cells are green, while dead cells are red. (G) IC50 values were determined to assess sensitivity of various organoids to carboplatin