Fig. 5

CircRAD23B/miR-1287-5p/YBX1 axis regulated proliferation and carboplatin resistance of ovarian cancer. (A) The expression of YBX1 mRNA in different transfected ovarian cancer cell lines. (B) The expression of YBX1 protein in different transfected ovarian cancer cell lines. (C) Representative images of colony formation. (D) Statistical analysis of colony formation. (E) CCK-8 assay in HO8910 and A2780 cells transfected with pLV-circRAD23B, vector, miR-1287-5p mimics and pLV-circRAD23B + miR-1287-5p mimics. (F) Representative images of EDU exprements and statistical analysis of EDU exprements. Red represents EDU staining, blue represents DAPI staining. (G) Apopotosis rate was analysed by flow cytometry after carboplatin treatment. (H) The IC₅₀ of carboplatin in HO8910 and A2780 cells transfected with pLV-circRAD23B, vector, miR-1287-5p mimics and pLV-circRAD23B + miR-1287-5p mimics. Graph represents mean ± SD; **p < 0.01, and ***p < 0.001