Fig. 2

EN1 is highly expressed in SACC, which is correlated with metastasis prognosis. A–F Haematoxylin and eosin (A–C) and EN1 immunohistochemical staining showing positive EN1 expression (D–F) in different SACC histological types. Magnification, 100×, scale bar, 200 μm. G–H Kaplan–Meier analysis of distant metastasis-free survival (G) and overall survival (H) of SACC cases with high or low EN1 expression. I–K EN1 mRNA and protein expressions assessed by qPCR (I) and immunoblotting (J) in the two SACC cell lines. Quantitative results of the relative expression of EN1 protein detected by western blot (K). L Immunofluorescence staining for EN1 in SACC-83 and SACC-LM cell lines. *P < 0.05, **P < 0.01, ***P < 0.001, and ns (not significant) by t-test