Fig. 3

EN1 promotes SACC cell invasion and migration through EMT. A–D qRT-PCR (A, B) and immunoblotting (C–F) after EN1 upregulation (A, C, E) and downregulation (B, D, F). Quantitative results of the relative expression of EN1 protein detected by western blot (E–F). G–I Transwell assays of the invasion and migration ability of EN1-overexpressing and control SACC-83 cells. H–I are the quantifications of the data shown in G. J–L Transwell assay of the invasion and migration ability of EN1-knockdown and control SACC-LM cells. K–L are the quantifications of the data shown in (J). M–N Wound-healing assays were performed to assess the migration abilities of the cells treated as indicated. O–R Proliferation abilities of the cells transfected as indicated were evaluated by CCK8 (O, P) and (Q, R) colony formation assays. S–T E-cadherin, N-cadherin, and Vimentin protein levels were analysed using western blotting. Quantitative results of the relative expression of protein detected by western blot (U-V). *P < 0.05, **P < 0.01, ***P < 0.001, and ns (not significant) by t-test