Fig. 4

EN1 promotes SACC invasion and migration in vivo. A–B EN1 protein levels were examined in EN1-knockout SACC-LM cells by western blotting. Quantitative results of the relative expression of EN1 protein detected by western blot (B). C Fluorescence imaging of mouse lung tissue after injection of EN1-overexpressing (EN1-ove) and negative control SACC-83 cells was performed to evaluate lung tumour metastasis. D Formation of lung metastases in mice after injection of EN1-ove or negative control SACC-83 cells. E Formation of lung metastases in mice after injection of EN1-KO or negative control SACC-LM cells. F–I Haematoxylin and eosin staining show lung metastases in mice injected with the indicated cells. Scale bar, 100 μm. J–M Quantification of metastatic tumour nodules and metastatic burden in lung sections from the indicated groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ns (not significant) by t-test