Fig. 1
Evaluation of in vitro metastatic potential in PC-3 and 22Rv1 cells after H19 silencing or overexpression. PC-3M-luc2 (left) and 22Rv1-luc (right) cells were subjected to lentiviral transduction to obtain stable H19 silencing (siH19) compared to scramble vector (Vector) and H19 overexpression (oeH19) compared to empty vector (EV). (A) H19, E-cadherin (CDH1), and β4 integrin (ITGB4) RNAs were assessed by qPCR. Data, plotted as fold change vs. Vector or EV (dashed line), represent the mean ± SEM of 4 independent experiments (white dots). *P < 0.05 vs. Vector or EV. (B) Proliferation assay at different time points. Cells were monitored using the IncuCyte live cell analysis system. Cell confluence was calculated from raw data images. Data represent the mean ± SEM of 3 independent experiments performed in triplicate. *P < 0.05 vs. Vector; # P < 0.05 vs. PC-3 or 22Rv1. (C) Trans well Cell invasion assay after 16 h. Upper panel: representative phase-contrast microscopic images of invading cells under 20X magnification (bright field). Scale bar: 20 μm. Lower panel: number of invading cells. Data plotted as fold change vs. mean vector represent the mean ± SEM of 4 independent experiments (white dots). *P < 0.05