Fig. 2

Efficacy of GSK-J4 on cell adhesion molecules expression, proliferation, and invasiveness in siH19 cells. H19-silenced (siH19) or control (Vector) PC-3M-luc2 (left) and 22Rv1-luc (right) cells were treated for 72 h with GSK-J4 demethylases inhibitor (1µM) or DMSO as control. (A) CDH1 and ITGB4 mRNA levels were assessed by qPCR. Data plotted as fold change vs. mean Vector + DMSO represent the mean ± SEM of 4 independent experiments (white dots). (B) Cell proliferation was monitored using the IncuCyte live cell analysis system. Upper: raw data pictures of cell confluence exported from the IncuCyte system after 48 h incubation; scale bar is indicated; Lower: Cell confluence was calculated from raw data images; data shown is a representative experiment of 4 biological replicates, each time point represent the mean of 4 samples. *P < 0.05 vs. Vector + DMSO; ^$ P < 0.05 vs. siH19 + J4. (C) Cell invasion by Trans well assay. Upper: representative phase contrast microscopic images of invading cells under 20X magnification (bright field). Scale bar: 20 μm. Lower: number of invading cells. Data plotted as fold change vs. mean Vector + DMSO represent the mean ± SEM of 4 independent experiments (white dots). *P < 0.05