Fig. 3

Effects of KDM6A and KDM6B silencing on cell adhesion molecules expression in siH19 cells. SiH19 and Vector cells were transfected with siRNA specific for KDM6A and KDM6B or scramble (NC1), and analysis was performed after 72 h. (A) CDH1 and ITGB4 mRNA levels were quantified by qPCR. Data are represented as mean ± SEM of fold change vs. mean Vector/NC1 cells of 4 independent experiments (white dots). *P < 0.05. (B) Representative E-Cad and β4-integrin western blot (left panels) and densitometry analysis (right panels) after KDM6A and KDM6B interfering. β-actin served as control. Molecular weight marker is indicated. Data are represented as mean ± SEM of fold change vs. mean Vector/NC1 cells of 4 independent experiments (white dots). *P < 0.05. (C) Enrichment of H3K27me3 (left) and recruitment of KDM6A/UTX and KDM6B/JMJD3 (right) on the promoter region of CDH1 and ITGB4 by ChIPs. IgG served as the negative control. Values represent the mean ± SEM of 4 independent experiments. Data are plotted as Relative enrichment relative to Input in Arbitrary Unit (A.U.) or fold vs. mean Vector. *P < 0.05 vs. Vector