Fig. 5

Metastatic dissemination of PC-3M-luc2 cells after H19 silencing. (A) Sequential in vivo imaging of metastatic tumor dissemination over time post intravenous injection of siH19 and control Vector cells in NGS mice in the presence or absence of GSK-J4 (50 mg/kg) or DMSO as control (n = 12 mice/group). (B) Representative ex vivo bioluminescence images in different dissemination sites (column, tibiae, lung, and liver). (C) Representative sections H&E-stained (left) and IHC for human cytokeratin CK8-18 (right) sections of the lung with Vector and siH19 cells. Scale bar: 50 μm. (D) Representative H&E-stained sections of bone metastasis under 10X and 20X magnification. Metastatic cells, bone, and cartilage, are indicated.