Fig. 7

Effects of GSK-J4 on Organotypic Slices Cultures. (A) Representative images of OSC after 72 h treatment with GSK-J4 (J4, 5µM) or DMSO as control. (B) Quantification of CDH1 and ITGB4 transcripts by qRT-PCR (total OSC n = 25). OSCs were divided into J4-responder or J4-non responder according to a 25% reduction upon treatment. Data plotted as fold change vs. DMSO (dashed line) represent mean +/-SEM, and white dots indicate a single OSC. *P < 0.05 vs. DMSO. (C) Apoptosis induction upon GSK-J4 treatment evaluated using Cell Death Detection ELISA Kit as described in Methods. Data are expressed as fold change vs. DMSO (n = 15). P < 0.05 vs. DMSO