Fig. 4

Gene expression of spatially transcriptomic-defined clusters in STAD. A The tissue sections were analyzed by spatial transcriptomics to identify clusters, and proper alignment with morphology was demonstrated through hematoxylin and eosin staining and cluster maps. B The spatial location and gene changes of ALKBH1, AQP1, and PECAM1 were visualized in different tissue sections using 10X Visium spatial gene expression. The variation of each gene in boxes i and ii was illustrated in an enlarged images. C A dot plot was used to show the levels of gene expression in different clusters. D A box plot was used to display alterations in the expression of three genes