Fig. 1
From: Genome-wide CRISPR screen identifies ESPL1 limits the response of gastric cancer cells to apatinib
Schematic of functional screening by CRISPR/Cas9 SAM pooled library and apatinib treatment. (a) Gastric cancer cell lines: AGS, HGC-27, NCI-N87 and BGC823 were treated with different concentrations of apatinib (0, 0.5, 2, 10, 40 μg/mL) for 24 h and the cell viability was observed; b) Schematic of apatinib-resistant GC cells construction for high-throughput sequencing analysis; c) Optical microscopic images of GC cells transfected with lentiviral sgRNA library and treated with apatinib for two rounds