Fig. 3
From: Genome-wide CRISPR screen identifies ESPL1 limits the response of gastric cancer cells to apatinib
The overexpression of the candidate genes increases apatinib resistance in GC cell lines. a CCK8 assay was used to detect cell viability for AGS and HGC-27 cells with individual gene overexpression, relative growth rate was calculated based on the absorbance in NC; b, c Transwell assays clearly revealed the invasion cells of AGS and HGC-27 with individual gene overexpression at 24 h after treated with 10 μg/mL apatinib for 48 h; d, e Apoptosis assay was conducted to detect the effect of candidate gene overexpression on apatinib resistance in AGS and HGC-27 cells after treated with 10 μg/mL apatinib for 48 h. Statistical significance was determined by One-way ANOVA. Data were represented as means ± SD.**P < 0.01, ***P < 0.001 compared with the NC group