Fig. 7

Inhibition of MDM2 rescued the sensitivity of ESPL1 overexpressing GC cells to apatinib. a The MDM2 siRNA was used to inhibit the expression of MDM2 and western blots was used to detect MDM2 protein levels in AGS and HGC27 cells (left), the Image J software was used for quantitative analysis (right); b Western blots was used to detect ESPL1 protein levels in MDM2 low-expression AGS and HGC27 cells(left), the Image J software was used for quantitative analysis (right); c Co-IP was used to detect the interaction between MDM2 and ESPL1 protein; d Time-course cell proliferation experiments of AGS-NC, AGS-ESPL1-OE, AGS-ESPL1-OE + siMDM2 cells under treatment of 10 μg/mL apatinib were detected by CCK8 at 4 h, 24 h, 48 h, 72 h; e Transwell assays clearly revealed the invasion cells in AGS-NC, AGS-ESPL1-OE, and AGS-ESPL1-OE + siMDM2 group after treated with 10 μg/mL apatinib for 48 h; f Apoptosis assays showed the proportion of apoptotic cells in AGS-NC, AGS-ESPL1-OE and AGS-ESPL1-OE + siMDM2 group after treated with 10 μg/mL apatinib for 48 h. Statistical significance was determined by One-way ANOVA. Data were represented as means ± SD. ***P < 0.001 compared with the NC group