Table 3 Primers sequences for q-PCR
From: Genome-wide CRISPR screen identifies ESPL1 limits the response of gastric cancer cells to apatinib
Gene symbol | Accession number | Product size | Primer sequences |
|---|---|---|---|
β-actin | HQ154074 | 185 bp | F: TGGCACCAGCACAATGAA |
R: CTAAGTCATAGTCCGCCTAGAAGCA | |||
MCM2 | NM_004526 | 162 bp | F: GCCATGCCCAACACGTATG |
R: GCCTGTCGCCATAGATTCTTTC | |||
CCND3 | NM_001136017 | 239 bp | F: GCCCCTGACCATCGAAAAAC |
R: TGGCAAAGGTATAATCTGTAGCAC | |||
PLK1 | NM_005030 | 117 bp | F: GTACGGCCTTGGGTATCAGC |
R: GTGCCGTCACGCTCTATGTA | |||
TCF7 | NM_001134851 | 182 bp | F: GCTGCCATCAACCAGATCCT |
R: GTGGATTCTTGGTGCTTTTCCC | |||
PRICKLE1 | NM_001144881 | 189 bp | F: TGAGTGCACAGAAGCTGAGG |
R: TGTGCATGGTCCACACCAAT | |||
VANGL1 | NM_138959 | 123 bp | F: AGCCTGGGACACCTGAGTATC |
R: CATATGCTTGGCTGCTCGGA | |||
ESPL1 | NM_012291 | 225 bp | F: TCAAAGAGTATGGGGCCTCG |
R: CCCCATGCCCTGCATAGATA |