Fig. 4
From: IGFBP3 induces PD-L1 expression to promote glioblastoma immune evasion
IGFBP3 induces immunosuppression in in-vitro co-culture system. A CCK-8 assay was used to analysis the viability of Jurkat cells after co-cultured with IGFBP3 overexpressing cells. B, C QRT-PCR was performed to examine the mRNA expression of IL2 (B) or IFN-γ (C) in Jurkat cells after co-cultured with IGFBP3 overexpressing cells. D, E ELISA was used to measure the levels of IL-2 (D) and IFN-γ (E) in the supernatants of Jurkat cells after co-cultured with IGFBP3 overexpressing cells. F Annexin V-PE apoptosis assay was used to determine apoptosis of Jurkat cells after co-cultured with IGFBP3 overexpressing cells. G Statistical chart of apoptotic cells. H Immunoblotting was performed to examine the caspase3 expression in Jurkat cells after co-cultured with IGFBP3 overexpressing cells. Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001, Student t test