Fig. 1

Cytotoxic effects of AZD4547 in epithelial ovarian cancer (EOC) cell lines. (A) Expression levels of FGFRs in EOC cell lines were analyzed by western blot. (B) EOC cells were treated with AZD4547 for the indicated times, and cell viability was evaluated by MTT assay. (C) Colony formation assay was performed with EOC cell lines. AZD4547 was incubated for 8 days until colony was formed. Colony was stained and counted or stained colonies were measured by the optical density at 560 nm after extraction of colonies as described in “Methods”