Table 3 Primer sequences involved
Gene | Forward primer | Reverse primer |
|---|---|---|
SNORD19B | TGGTTGAAATATGATGAGTGTAC | GAAATCAGAGTTGGATCTTGTAA |
SNORA25 | TGAGGCTGTGAAACCCAGAG | GCACCAAACATTAGGAGTGC |
SNORA65 | TCTCTGTTGGCTGGTGCAAT | TGCTTTCGGCACAGAGTCAT |
SNORD15A | GGGGATGTTCTCTTTGCCCA | AGGGCTCTTTAAACTGTGCCA |
SNORD2 | ATGGCAATCATCTTTCGGGAC | CAAGTGATCAGCAATGAGTATTCT |
SNORD12B | TTTTTCCCCGACAGATCGAC | GCTCAAGCTGGCATATCAGAC |
SNORA64 | TCGGCTCTGCATAGTTGCAC | TGCACCCCTCAAGGAAAGAG |
SNORA70B | AGCCAATTAAGCCGACTGAGTTCC | GTCCCTTAGAGCAACCCATACAACC |
SNORA24 | TGTCAAGTGTGGCAGTCTCC | ATGGTGACAGCTTTGCTGGT |
U76 | GCCACAATGATGACAGTTTATTTGC | GCCTCAGTTAAGATAATGGTGGT |
U44 | GAGAATACTCATTGCTGATCACTTG | universal primer |
SNORD5 | TCAGATGATGAATTTAACTGTTCAACTG | AGTTTTAGTTCATGCCCGTTATCA |
SNORA69 | AGGTTGCAATTACAGTGCTTCA | CACGGCTTTTCTTTCAGCAGG |
SNORD41 | GGAAGTGATGACACCTGTGACT | CAGCCAGTACGAATACGCGA |
SNORD16 | TGCAATGATGTCGTAATTTGCGTC | TCGTCAACCTTCTGTACCAGCT |
SNORA73B | AGGCTCTGTCCAAGTGGCATAGG | CGAGGCCCAGCTTCATCTTCAAC |
SCARNA4 | CTGGAGGACTAAGAAGGCTGA | GAAGGCTGCTCTCTCCAACC |
SNORD49B | TGATACTTGTAATAGGAAGTGCCG | CAGATAGACATTAGACGTCGTCAG |
U6 | TGGAACGCTTCACGAATTTGCG | GGAACGATACAGAGAAGATTAGC |