Fig. 2

LINC00894 inhibits the lymphangiogenesis of vascular endothelial cells and the proliferation of PTC cells. (A) Predicting the coding capability of LINC00894 using the CPC2 database. (B) Bioinformatics analyses predicted the subcellular distribution of LINC00894 in various cells. (C) Subcellular localization of LINC00894 in KTC-1 and BCPAP cells. The nuclear marker was U6, and the cytosol marker was GAPDH. (D) FISH analysis of the location of LINC00894 in the cytoplasm and nuclear fractions of PTC cells. (E) Verification of the overexpression efficiency of LINC00894 through qRT‒PCR. F and H. Effect of altered LINC00894 expression in PTC cells on HUVEC tube formation. I and K. For LINC00894-overexpressing cells, a colony formation assay was used to evaluate PTC cell proliferation. L and M. For LINC00894-overexpressing cells, the CCK-8 assay was used to evaluate PTC cell viability. N and O. For LINC00894-overexpressing cells, the EdU assay was used to evaluate cell proliferations. **P < 0.01, ***P < 0.001, ****P < 0.0001