Fig. 7

Regulatory role of NDUFAF6 on NRF2 and its impact on PD-L1 expression, cell proliferation, and apoptosis in triple-negative breast cancer. A, B Western blot was used to examine the impact of sh-NDUFAF6 and ov-NDUFAF6 treatments on the protein levels of PD-L1 in breast cancer xenografts, as well as their quantitative analysis (n = 5). C, D Western blot was performed to assess the influence of NRF2 overexpression on the protein levels of PD-L1 and NDUFAF6, along with their quantitative analysis (n = 5). E Physical images of xenografts formed by ov-NC group and ov-NRF2 group (n = 5). F, G Impact of NRF2 overexpression on the volume and weight of MCF-7 cell xenografts (n = 5). H, I Immunohistochemical detection of changes in the Ki-67 positivity rate in xenograft tissues and its quantitative analysis (n = 5). J, K TUNEL assay detection of the impact of NRF2 overexpression on the apoptosis rate of MCF-7 cell xenografts and its quantitative analysis (n = 5). **Indicates P < 0.01