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Fig. 5 | Cancer Cell International

Fig. 5

From: An estrogen-regulated long non-coding RNA NCALD promotes luminal breast cancer proliferation by activating GRHL2

Fig. 5

LncRNA NCALD interacts with the ERα to modulate the transcription of GRHL2. Knockdown of lncRNA NCALD or ESR1 inhibited expression of GRHL2. However, overexpression of ESR1 did not reverse the level of GRHL2 induced by knockdown of lncRNA NCALD (n = 3, ***P < 0.001) (panel a). Concomitant overexpression of ESR1 and lncRNA NCALD led to an upregulation of GRHL2 (n = 3, ***P < 0.001) (panel b). Knocking down ESR1 or lncRNA NCALD resulted in the downregulation of GRHL2 protein levels. Only when lncRNA NCALD and ESR1 were simultaneously overexpressed, GRHL2 expression was promoted (n = 3, *P < 0.05) (panel c). The ChIP-qPCR assay demonstrated that the knockdown of lncRNA NCALD significantly inhibited ERα binding at the GRHL2 promoter (n = 3, ***P < 0.001) (panel d)

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Cancer Cell International

ISSN: 1475-2867

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