Fig. 9

SEMA3C knockdown inhibited cell proliferation, migration, and invasion in TSCC cells. c, d qRT-PCR for the mRNA expressions of SEMA3C in SCC9 (c) and SCC25 (d) transfected with shRNA of SEMA3C. a, b Cell proliferation was remarkably suppressed when endogenous SEMA3C was silenced as measured by CCK-8 viability assay. e, f The potentials of colony formation were significantly inhibited in SEMA3C-depleted cells as compared to control (shNT). g–j The migration (g, h) and invasion (i, j) abilities were significantly reduced in shSEMA3C-transfected cells in wound healing and transwell assays, respectively. Data shown here were mean ± SD from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001