Fig. 5
From: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression
PRDX3 regulates MMP1 via MAPK. (A) Multi-pathway reporter array showed the transcriptional activities change of AP-1 in PRDX3-OE group (refer to Supplementary Figure for full investigated pathways). Relative luciferase activity was denoted as the ratio of firefly luciferase to renilla luciferase values. (B) Representative western blots showing protein expression of p-c-Jun, total c-Jun. Lamin B1 was used as nuclear loading control while GAPDH was used as cytoplasmic fraction control. (C) Human phospho-MAPK proteome profiler array demonstrates PRDX3 induced phosphorylation of MAPK and other serine/threonine kinases. Data is representative of a single experiment out of two experiments done. (D) Graph bars depicted the relative protein expression levels of several phosphorylated proteins in PRDX3-OE samples. (E) Treatment with SCH772984, an ERK inhibitor, for 24 h inhibited MMP1 expression in PRDX3-overexpressing MDA-MB-231 cells. Each bar represents mean ± SEM, **P < 0.01, ***P < 0.001, ****P < 0.0001