Fig. 1From: NY-ESO-1-specific T cell receptor-engineered T cells and Tranilast, a TRPV2 antagonist bivalent treatment enhances the killing of esophageal cancer: a dual-targeted cancer therapeutic routeNGS-quality assessment of αβTCR library. a schematic representation of the original gene arrangement of αβTCR variable chain. A linker separates αTCR and βTCR. b schematic representation of separate αTCR or βTCR indexed with molecular identifiers. c–f TCR DNA quality and size characterization. Fragmented αTCR and βTCR Trap DNA of about 480 bp (c). αTCR and βTCR DNA smear integrity data (d). Electropherogram diagram showing the size distribution range of αTCR and βTCR DNA libraries as migration time versus intensity (relative fluorescence unit, RFU) spectrum (e). Evaluation of the quality of bases read for each αTCR and βTCR library (f). g diversity of αTCR and βTCR libraries. Base numbers 0 to 55 and 251 to 300 represent molecular identifiers, base numbers 56 to 250 and 301 to 500 represent the variable (V) region of the αTCR and βTCRBack to article page