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Table 1 Primer pairs used to assess the quality of synthesized cDNA

From: NY-ESO-1-specific T cell receptor-engineered T cells and Tranilast, a TRPV2 antagonist bivalent treatment enhances the killing of esophageal cancer: a dual-targeted cancer therapeutic route

Housekeeping gene

Oligo 1 (5ʹ to 3ʹ)

Oligo 2 (5ʹ to 3ʹ)

Product length (bp)

ACTB

GGCTGTGCTATCCCTGTACG

CTTGATCTTCATTGTGCTGGGTG

574

B2M

GCTCGCGCTACTCTCTCTTT

CACGGCAGGCATACTCATCT

278

GAPDH

GACCACAGTCCATGCCATCA

GTCAAAGGTGGAGGAGTGGG

364

  1. PCR amplification oligos for actin beta (ACTB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and beta-2-microglobulin (B2M) utilizing cDNA as template
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Cancer Cell International

ISSN: 1475-2867

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